Honestly, I'm not sure if there are that big of gains of having separate drives, but I do personally have several drives in my PC. More so just because I started with this much space.. it wasn't enough.. I bumped up adding another drive, and so on and so on. In my own PC, I have O/S and some VSTs on a Samsung 960 Evo (NVMe), my Reaper projects/Renders qand some VSTs/libraries on a 1TB Crucial P1 (NVMe)... some non-music related stuff on a 1TB Samsung 980 (NVMe), and some VSTs/libraries on regular SATA 3 drives (a Samsung 840 Pro and a Seagate Barracuda 120). I have a lot of crap. lol Lots of Kontakt libraries I've collected over the time, for example.
With what you have, you'll probably want your O/S sitting on the SSD. 250GB is a good size for Windows, plus some other stuff. Just be careful about how much "other stuff" you add in there, as things can quickly add up.. but you should be good with 250. Reaper can sit there too.
As far as mechanical drives, I personally dislike them, but see how things go. Your most likely issue with a mechanical drive is it will take a bit longer to render to it, and it will take a bit longer to read large libraries from it. YMMV though, depending on what you're doing. In a perfect world, all your data would be sitting on NVMe SSDs (or even SATA3 SSDs), but we only have so much money to work with, unfortunately! lol
I don't really think there's much (if any) advantage to isolating everything as you described though, especially song files/audio from samples/libraries, but I do like to keep all those things separated from where the O/S is located (even if just by partitioning). Reason being, if you reformat/reinstall Windows, you don't lose all that other stuff.
If you had several drives of the same speed, I'd think the average Reaper user would be over-thinking things by worrying about isolating stuff on different drives. But in your case, I think installing the O/S and Reaper and a few things in need of zippier speeds you can fit on it would be good.
SSDs are fairly cheap nowadays. Ideally you dump the mechanical drives except for longer-term storage (backups, etc.). The basic Samsung 980 1TB (NVMe) is only $110 right now, for example. That's the non-Pro, but I have one of these and it's a super fast drive. That's even cheaper than the Samsung 870 Evo 1TB (SATA III). Crazy.
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My Rig (also serves as my gaming PC): MSI Mag X570 Tomahawk Mobo, Ryzen R9 3900X, 32GB RAM, Samsung 960 Evo 500gb NVMe, Crucial 1TB NVMe, NVidia RTX 2080 Super, Arturia Minifuse 2, Nektar Impact LX25+ MIDI Controller Keyboard.
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