not if it does not exist as saved on the HD
for the future, it is good to store all the audio for a project in a folder called Audio that is right in the project... you can do this if you name a folder Audio in the Project settings and then for each project do a one time Save as, and check the boxes at the bottom to copy all audio into the project....
...after that first Save as you can just use Save to keep updating the project
secondly, if you are going to destructively gate or other FX to a wav file, first make a copy of that on a new track and mute the original and deal with the copy... that way you'll always have both
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