I don’t want to confuse the OP issue here, but I think that’s sorted, so I’m gonna go off on this little tangent about loopback and “calibration” that really has nothing to do with what they were seeing, but is a thing people might run into.
The thing is that many interfaces, including the most common ones from big manufacturers like Presonus, Focusrite, etc, are NOT matched from input to output. Their maximum input level is different from their maximum output level in almost every case. Now you’d kind of expect a mic input to have some gain over the corresponding line input (on my Tascam it’s a minimum of 6db hotter) and most instrument inputs are 9-10db louder than the line in, but one would sort of expect the line ins to be essentially unity gain through to the output so that, say, +4dbu input would be the same +4dbu at the output as long as everything along the way is set to 0db gain. That will almost never be true, though. Very often the output will be significantly louder. I’ve seen interface specs where the maximum input level is like +8dbu while the output goes to like +18dbu or more. Those both correspond to 0dbFS, which means there’s 10db or gain when you thought you had everything at unity!
This only really matters if it matters, like if you’re trying to do hybrid mixing with hardware as inserts or other things like that. Reamping is an example. You’d kind of like for the signal going back out to the amp to be the same it would see from the guitar, but even if you use a good active buffer and record through the line in, it’s going to pound the amp unless you compensate somewhere along the way. That’s not so bad IF your manufacturer actually provides the appropriate specs, but with some it’s pretty tough to find that information.